Evaluation of diagnostic methods for the Detection of Bovine Coronavirus and Rotavirus in feces of diarrheic calves

The purpose of the present study was to evaluate efficacy Enzyme-Linked Immunosorbent Assay (ELISA), immunochromatographic (ICG), and reverse transcription-polymerase chain reaction (RT-PCR) methods for detection rotavirus (RV) bovine coronavirus (BCV). Feces samples were collected from 90 diarrhoeic calves (male female) up one month age immune response against RV BCV infection assessed by using ELISA (Ag Ab), ICG, RT-PCR. To determine performance accuracy each diagnostic method in comparison gold standard method, different statistical tests including receiver operating characteristic curve (ROC), concordance correlation used. Results shown prevalence RV+BCV according RT-PCR equal 8.89 (95% CI: 6.64-10.07), 14.44 11.23-6.90), 2.22 0.89-3.72), respectively. best agreement highest sensitivity specificity obtained between AgELISA (100% 94.3%), then AbELISA 94.3%) second-best test also ICG less accurate identifying BCV, but a good techniques observed. put it nutshell, our results suggest that assay may improve ability diagnose infections accurately quickly. Promoting rapid IGG kit with higher early diagnosis cause diarrhoea plays an important role its therapeutic regimes, management protocols, control procedures, is preferred due more precise results.